25,000 step-by-step techniques, procedures, and practical overviews
Every Step Counts
The Current Protocols collection includes over 25,000 step-by-step techniques, procedures, and practical overviews that provide researchers with reliable, efficient methods to ensure reproducible results and pave the way for critical scientific discovery. With its emphasis on carefully curated, highly edited methods rich in detail, practical advice, and troubleshooting, Current Protocols enables researchers to advance their research with an efficiency of time and resources.
- Bioinformatics
- Cell Biology
- Chemical Biology
- Cytometry
- Essential Lab Techniques
- Food Analytical Chemistry
- Human Genetics
- Immunology
- Magnetic Resonance Imaging
- Microbiology
- Molecular Biology
- Mammalian Systems & Disease Models
- Neuroscience
- Nucleic Acid Chemistry
- Pharmacology
- Plant Biology
- Protein Science
- Stem Cell Biology
- Toxicology
Browse Articles
Workflow4Metabolomics (W4M): A User‐Friendly Metabolomics Platform for Analysis of Mass Spectrometry and Nuclear Magnetic Resonance Data
- Current Protocols
-  14 February 2025
Monitoring Mouse Surface Temperature During Stress with a Thermal Camera: A Low‐Cost Infrared Videography System for Evaluating Murine Metabolism
- Current Protocols
-  13 February 2025
Efficient Site‐Directed Mutagenesis Mediated by Primer Pairs with 3’‐Overhangs
- Current Protocols
-  13 February 2025
Minimal Component, Protein‐Free, and Cost‐effective Human Pluripotent Stem Cell Cardiomyocyte Differentiation
- Current Protocols
-  11 February 2025
Porphyria Diagnostics Part 2: Essential Biochemical Testing for Diagnosis of the Porphyrias
- Current Protocols
-  10 February 2025
Zebrafish Model for Thrombosis and Brain‐Behavior Studies
- Current Protocols
-  10 February 2025
Creating Optimal Western Blot Conditions for OPA1 Isoforms in Skeletal Muscle Cells and Tissue
- Current Protocols
-  5 February 2025
Optimizing In Situ Proximity Ligation Assays for Mitochondria, ER, or MERC Markers in Skeletal Muscle Tissue and Cells
- Current Protocols
-  5 February 2025
Analyzing Pathophysiology and Immune Cells and Their Cytokines and Mediators in Precision‐Cut Slices of the Murine Lung
- Current Protocols
-  28 January 2025
Streptozotocin‐Induced Diabetic Models in Mice and Rats
- Current Protocols
-  27 April 2021
Working with Worms: Caenorhabditis elegans as a Model Organism
- Current Protocols Essential Laboratory Techniques
-  3 September 2019
Synthetic DNA Assembly Using Golden Gate Cloning and the Hierarchical Modular Cloning Pipeline
- Current Protocols in Molecular Biology
-  11 March 2020
Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS‐CoV‐2
- Current Protocols in Microbiology
-  31 May 2020
Fluorescence Polarization (FP) Assays for Monitoring Peptide‐Protein or Nucleic Acid‐Protein Binding
- Current Protocols in Chemical Biology
-  1-15
-  1 December 2009
Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9
- Current Protocols in Molecular Biology
-  26 July 2019
The GeneCards Suite: From Gene Data Mining to Disease Genome Sequence Analyses
- Current Protocols in Bioinformatics
-  1.30.1-1.30.33
-  20 June 2016
Gene Set Knowledge Discovery with Enrichr
- Current Protocols
-  29 March 2021
Using SPAdes De Novo Assembler
- Current Protocols in Bioinformatics
-  19 June 2020
From FastQ Data to High‐Confidence Variant Calls: The Genome Analysis Toolkit Best Practices Pipeline
- Current Protocols in Bioinformatics
-  11.10.1-11.10.33
-  15 March 2018
Comparative Protein Structure Modeling Using MODELLER
- Current Protocols in Bioinformatics
-  5.6.1-5.6.37
-  20 June 2016
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
- Current Protocols in Cell Biology
-  3.22.1-3.22.29
-  1 April 2006
ATAC‐seq: A Method for Assaying Chromatin Accessibility Genome‐Wide
- Current Protocols in Molecular Biology
-  21.29.1-21.29.9
-  5 January 2015
Chlorophylls and Carotenoids: Measurement and Characterization by UV-VIS Spectroscopy
- Current Protocols in Food Analytical Chemistry
-  F4.3.1-F4.3.8
-  1 November 2001
