25,000 step-by-step techniques, procedures, and practical overviews
Every Step Counts
The Current Protocols collection includes over 25,000 step-by-step techniques, procedures, and practical overviews that provide researchers with reliable, efficient methods to ensure reproducible results and pave the way for critical scientific discovery. With its emphasis on carefully curated, highly edited methods rich in detail, practical advice, and troubleshooting, Current Protocols enables researchers to advance their research with an efficiency of time and resources.
- Bioinformatics
- Cell Biology
- Chemical Biology
- Cytometry
- Essential Lab Techniques
- Food Analytical Chemistry
- Human Genetics
- Imaging
- Immunology
- Magnetic Resonance Imaging
- Microbiology
- Molecular Biology
- Mammalian Systems & Disease Models
- Neuroscience
- Nucleic Acid Chemistry
- Pharmacology
- Plant Biology
- Protein Science
- Stem Cell Biology
- Toxicology
Browse Articles
Epigenetic Histone Deacetylases Activity Assay in the Brain and Peripheral Organ Tissues
- Current Protocols
- 11 June 2025
Methods to Observe Plant Tissue Colonization by Fusarium oxysporum
- Current Protocols
- 10 June 2025
From Genetic Association to Therapeutic Target: A Pipeline for Pleiotropic Gene Prioritization
- Current Protocols
- 9 June 2025
Quantitative Assessment of Mitochondrial Volumetric Transitions in Arabidopsis thaliana
- Current Protocols
- 4 June 2025
Peptide–Oligonucleotide Conjugates: Catalytic Preparation in Aqueous Solution or On‐Column
- Current Protocols
- 3 June 2025
Simple and Efficient Transformation and Gene Editing of Marchantia polymorpha Spores
- Current Protocols
- 27 May 2025
Co‐Expression and Purification of the Complex of Single‐Chain H2B‐H2A.Z and Histone Chaperone YL1
- Current Protocols
- 27 May 2025
Issue Information
- Current Protocols
- 27 May 2025
Cover Image, Volume 5, Issue 5
- Current Protocols
- 27 May 2025
Graphical Abstract
The cover image is based on the article Purification of recombinant histones and mononucleosome assembly by Jingjun Hong et al., https://doi.org/10.1002/cpz1.70155.
Working with Worms: Caenorhabditis elegans as a Model Organism
- Current Protocols Essential Laboratory Techniques
- 3 September 2019
Discriminating Between Apoptosis, Necrosis, Necroptosis, and Ferroptosis by Microscopy and Flow Cytometry
- Current Protocols
- 19 December 2023
Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS‐CoV‐2
- Current Protocols in Microbiology
- 31 May 2020
Synthetic DNA Assembly Using Golden Gate Cloning and the Hierarchical Modular Cloning Pipeline
- Current Protocols in Molecular Biology
- 11 March 2020
Fluorescence Polarization (FP) Assays for Monitoring Peptide‐Protein or Nucleic Acid‐Protein Binding
- Current Protocols in Chemical Biology
- 1-15
- 1 December 2009
The GeneCards Suite: From Gene Data Mining to Disease Genome Sequence Analyses
- Current Protocols in Bioinformatics
- 1.30.1-1.30.33
- 20 June 2016
Gene Set Knowledge Discovery with Enrichr
- Current Protocols
- 29 March 2021
From FastQ Data to High‐Confidence Variant Calls: The Genome Analysis Toolkit Best Practices Pipeline
- Current Protocols in Bioinformatics
- 11.10.1-11.10.33
- 15 March 2018
Comparative Protein Structure Modeling Using MODELLER
- Current Protocols in Bioinformatics
- 5.6.1-5.6.37
- 20 June 2016
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
- Current Protocols in Cell Biology
- 3.22.1-3.22.29
- 1 April 2006
ATAC‐seq: A Method for Assaying Chromatin Accessibility Genome‐Wide
- Current Protocols in Molecular Biology
- 21.29.1-21.29.9
- 5 January 2015
Chlorophylls and Carotenoids: Measurement and Characterization by UV-VIS Spectroscopy
- Current Protocols in Food Analytical Chemistry
- F4.3.1-F4.3.8
- 1 November 2001
Using RepeatMasker to Identify Repetitive Elements in Genomic Sequences
- Current Protocols in Bioinformatics
- 4.10.1-4.10.14
- 1 March 2009
Best of Current Protocols
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Fluorescence Polarization (FP) Assays for Monitoring Peptide‐Protein or Nucleic Acid‐Protein Binding
Flow Cytometry Method Validation Protocols
Preparation, Culture, and Analysis of Amniotic Fluid Samples
Trypan Blue Exclusion Test of Cell Viability
ATAC‐seq: A Method for Assaying Chromatin Accessibility Genome‐Wide
Gram Staining
Cre‐loxP‐Mediated Recombination: General Principles and Experimental Considerations
RNA‐Seq for Bacterial Gene Expression
Streptozotocin‐Induced Diabetic Models in Mice and Rats
BioID: A Screen for Protein‐Protein Interactions
FACS‐Mediated Isolation of Neuronal Cell Populations From Virus‐Infected Human Embryonic Stem Cell-Derived Cerebral Organoid Cultures
Behavioral Phenotyping for Autism Spectrum Disorders in Mice
