25,000 step-by-step techniques, procedures, and practical overviews
Every Step Counts
The Current Protocols collection includes over 25,000 step-by-step techniques, procedures, and practical overviews that provide researchers with reliable, efficient methods to ensure reproducible results and pave the way for critical scientific discovery. With its emphasis on carefully curated, highly edited methods rich in detail, practical advice, and troubleshooting, Current Protocols enables researchers to advance their research with an efficiency of time and resources.
- Bioinformatics
- Cell Biology
- Chemical Biology
- Cytometry
- Essential Lab Techniques
- Food Analytical Chemistry
- Human Genetics
- Immunology
- Magnetic Resonance Imaging
- Microbiology
- Molecular Biology
- Mammalian Systems & Disease Models
- Neuroscience
- Nucleic Acid Chemistry
- Pharmacology
- Plant Biology
- Protein Science
- Stem Cell Biology
- Toxicology
Browse Articles
Synthesis of Oligodeoxynucleotide Containing Pseudo‐Deoxycytidine and Its Triphosphate Derivative
- Current Protocols
-  17 March 2025
Expression and Purification of SARS‐related Spike Glycoproteins for Cryo‐EM Analysis
- Current Protocols
-  8 March 2025
A Physiologically Relevant In Vitro Model of Nonreplicating Persistent Mycobacterium tuberculosis in Caseum
- Current Protocols
-  8 March 2025
Optimized, Efficient Measurement of the Expression of Undifferentiated Stem Cell Markers in Human Induced Pluripotent Stem Cells (iPSCs) by Flow Cytometry
- Current Protocols
-  3 March 2025
Imaging the Intestinal Transcriptome With Multiplexed Error‐Robust Fluorescence In Situ Hybridization (MERFISH)
- Current Protocols
-  3 March 2025
Development, Characterization, and Therapeutic Utility of Paclitaxel‐Resistant Breast and Gastric Cancer In Vitro and In Vivo Models
- Current Protocols
-  3 March 2025
Visualization of Efavirenz‐Induced Lipid Alterations in the Mouse Brain Using MALDI Mass Spectrometry Imaging
- Current Protocols
-  26 February 2025
Engineering Base Changes and Epitope‐Tagged Alleles in Mice Using Cas9 RNA‐Guided Nuclease
- Current Protocols
-  25 February 2025
Development of Syngeneic Murine Glioma Models with Somatic Mismatch Repair Deficiency to Study Therapeutic Responses to Alkylating Agents and Immunotherapy
- Current Protocols
-  24 February 2025
Streptozotocin‐Induced Diabetic Models in Mice and Rats
- Current Protocols
-  27 April 2021
Working with Worms: Caenorhabditis elegans as a Model Organism
- Current Protocols Essential Laboratory Techniques
-  3 September 2019
Synthetic DNA Assembly Using Golden Gate Cloning and the Hierarchical Modular Cloning Pipeline
- Current Protocols in Molecular Biology
-  11 March 2020
Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS‐CoV‐2
- Current Protocols in Microbiology
-  31 May 2020
Fluorescence Polarization (FP) Assays for Monitoring Peptide‐Protein or Nucleic Acid‐Protein Binding
- Current Protocols in Chemical Biology
-  1-15
-  1 December 2009
Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9
- Current Protocols in Molecular Biology
-  26 July 2019
The GeneCards Suite: From Gene Data Mining to Disease Genome Sequence Analyses
- Current Protocols in Bioinformatics
-  1.30.1-1.30.33
-  20 June 2016
Gene Set Knowledge Discovery with Enrichr
- Current Protocols
-  29 March 2021
Using SPAdes De Novo Assembler
- Current Protocols in Bioinformatics
-  19 June 2020
From FastQ Data to High‐Confidence Variant Calls: The Genome Analysis Toolkit Best Practices Pipeline
- Current Protocols in Bioinformatics
-  11.10.1-11.10.33
-  15 March 2018
Comparative Protein Structure Modeling Using MODELLER
- Current Protocols in Bioinformatics
-  5.6.1-5.6.37
-  20 June 2016
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
- Current Protocols in Cell Biology
-  3.22.1-3.22.29
-  1 April 2006
ATAC‐seq: A Method for Assaying Chromatin Accessibility Genome‐Wide
- Current Protocols in Molecular Biology
-  21.29.1-21.29.9
-  5 January 2015
Chlorophylls and Carotenoids: Measurement and Characterization by UV-VIS Spectroscopy
- Current Protocols in Food Analytical Chemistry
-  F4.3.1-F4.3.8
-  1 November 2001

Using RepeatMasker to Identify Repetitive Elements in Genomic Sequences
- Current Protocols in Bioinformatics
-  4.10.1-4.10.14
-  1 March 2009