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The Current Protocols collection includes over 25,000 step-by-step techniques, procedures, and practical overviews that provide researchers with reliable, efficient methods to ensure reproducible results and pave the way for critical scientific discovery. With its emphasis on carefully curated, highly edited methods rich in detail, practical advice, and troubleshooting, Current Protocols enables researchers to advance their research with an efficiency of time and resources.

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Scope Expansion: Call for Proposals Current Protocols in Mouse Biology

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Modeling Malignant Mesothelioma in Genetically Engineered Mice

Yuwaraj Kadariya, Eleonora Sementino, Xiang Hua, Dietmar J. Kappes, Joseph R. Testa, 
https://doi.org/10.1002/cpz1.70086
PROTOCOL
Open access

A Simple Protocol for Visualization of RNA–Protein Complexes by Atomic Force Microscopy

Andrea Tripepi, Huma Shakoor, Petr Klapetek, 
https://doi.org/10.1002/cpz1.70084
PROTOCOL
Open access

Miniscope Imaging of Nucleus Accumbens Neural Activity in Freely Behaving Rats: Virus Injection, Gradient Index Lens Implantation, Recording Strategies, and Analytical Methods

Nicholas J. Beacher, Michael W. Wang, Matthew C. Broomer, Jessica Y. Kuo, Kayden A. Washington, Miranda Targum, Yan Zhang, Giovanni Barbera, Da-Ting Lin, 
https://doi.org/10.1002/cpz1.70090
PROTOCOL
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Generation of Retinal Ganglion Cells from Reprogrammed Keratocytes of Non‐Glaucoma and Glaucoma Donors

Shahna S. Hameed, Tasneem P. Sharma, 
https://doi.org/10.1002/cpz1.70091
PROTOCOL

Genetically Engineered and Implantable Mouse Brain Tumor Models: Characterization by Immunohistochemistry and Flow Cytometry

Apoorva Mirji, Gurveer Singh, Anzar A. Mujeeb, Brandon L. McClellan, YingXiang Li, Makayla Perez, Maria G. Castro, 
https://doi.org/10.1002/cpz1.70080
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Targeted C-to-T Base Editing in the Arabidopsis Plastid Genome

Issei Nakazato, Shin-ichi Arimura, 
https://doi.org/10.1002/cpz1.70075
PROTOCOL

Simple Isolation of Human Bone Marrow Adipose Tissue–Derived Mesenchymal Stem/Stromal Cells

Gülsena Tonyalı, Emine Kiliç, Bihter Muratoğlu, Esin Alpdündar-Bulut, Cansu Özdemir, Duygu Uçkan-Çetinkaya, 
https://doi.org/10.1002/cpz1.70081
PROTOCOL
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Searching and Using MobiDB Resource 6 to Explore Predictions and Annotations for Intrinsically Disordered Proteins

Maria Cristina Aspromonte, Federica Quaglia, Alexander Miguel Monzon, Damiano Clementel, Alessio Del Conte, Damiano Piovesan, Silvio C. E. Tosatto, 
https://doi.org/10.1002/cpz1.70077
ISSUE INFORMATION
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Issue Information

https://doi.org/10.1002/cpz1.70089

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Issue Information Issue 12, 2024

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Employment of a Newly Defined In Vitro Fertilization Protocol to Determine the Cytoskeletal Machinery, DNA Damage, and Subsequent DNA Repair Resulting from Endocrine Disruption by Hexavalent Chromium in Rat Metaphase II Oocytes

Liga Wuri, Paul W. Zarutskie, Joe A. Arosh, Sakhila K. Banu, 
https://doi.org/10.1002/cpz1.70060
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Streptozotocin‐Induced Diabetic Models in Mice and Rats

Brian L. Furman, 
https://doi.org/10.1002/cpz1.78
Open access

Working with Worms: Caenorhabditis elegans as a Model Organism

Philip M. Meneely, Caroline L. Dahlberg, Jacqueline K. Rose, 
https://doi.org/10.1002/cpet.35
Open access

BUSCO: Assessing Genomic Data Quality and Beyond

Mosè Manni, Matthew R. Berkeley, Mathieu Seppey, Evgeny M. Zdobnov, 
https://doi.org/10.1002/cpz1.323
Open access

Synthetic DNA Assembly Using Golden Gate Cloning and the Hierarchical Modular Cloning Pipeline

Sylvestre Marillonnet, Ramona Grützner, 
https://doi.org/10.1002/cpmb.115
Open access

Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS‐CoV‐2

Emelissa J. Mendoza, Kathy Manguiat, Heidi Wood, Michael Drebot, 
https://doi.org/10.1002/cpmc.105
Open access

Overview of Genotyping Technologies and Methods

Ingrid Kockum, Jesse Huang, Pernilla Stridh, 
https://doi.org/10.1002/cpz1.727
free access

Fluorescence Polarization (FP) Assays for Monitoring Peptide‐Protein or Nucleic Acid‐Protein Binding

Nathan J. Moerke, 
https://doi.org/10.1002/9780470559277.ch090102
Open access

Mouse and Rat Anesthesia and Analgesia

Sang Su Oh, Heather L. Narver, 
https://doi.org/10.1002/cpz1.995
Open access

Generating Single Cell–Derived Knockout Clones in Mammalian Cells with CRISPR/Cas9

Christopher J. Giuliano, Ann Lin, Vishruth Girish, Jason M. Sheltzer, 
https://doi.org/10.1002/cpmb.100
Open access

Optimizing PCR for Mouse Genotyping: Recommendations for Reliable, Rapid, Cost Effective, Robust and Adaptable to High‐Throughput Genotyping Protocol for Any Type of Mutation

Sylvie Jacquot, Nathalie Chartoire, Françoise Piguet, Yann Hérault, Guillaume Pavlovic, 
https://doi.org/10.1002/cpmo.65

The GeneCards Suite: From Gene Data Mining to Disease Genome Sequence Analyses

Gil Stelzer, Naomi Rosen, Inbar Plaschkes, Shahar Zimmerman, Michal Twik, Simon Fishilevich, Tsippi Iny Stein, Ron Nudel, Iris Lieder, Yaron Mazor, Sergey Kaplan, Dvir Dahary, David Warshawsky, Yaron Guan-Golan, Asher Kohn, Noa Rappaport, Marilyn Safran, Doron Lancet, 
https://doi.org/10.1002/cpbi.5

Gene Set Knowledge Discovery with Enrichr

Zhuorui Xie, Allison Bailey, Maxim V. Kuleshov, Daniel J. B. Clarke, John E. Evangelista, Sherry L. Jenkins, Alexander Lachmann, Megan L. Wojciechowicz, Eryk Kropiwnicki, Kathleen M. Jagodnik, Minji Jeon, Avi Ma'ayan, 
https://doi.org/10.1002/cpz1.90

Using SPAdes De Novo Assembler

Andrey Prjibelski, Dmitry Antipov, Dmitry Meleshko, Alla Lapidus, Anton Korobeynikov, 
https://doi.org/10.1002/cpbi.102

From FastQ Data to High‐Confidence Variant Calls: The Genome Analysis Toolkit Best Practices Pipeline

Geraldine A. Van der Auwera, Mauricio O. Carneiro, Christopher Hartl, Ryan Poplin, Guillermo del Angel, Ami Levy-Moonshine, Tadeusz Jordan, Khalid Shakir, David Roazen, Joel Thibault, Eric Banks, Kiran V. Garimella, David Altshuler, Stacey Gabriel, Mark A. DePristo, 
https://doi.org/10.1002/0471250953.bi1110s43

Comparative Protein Structure Modeling Using MODELLER

Benjamin Webb, Andrej Sali, 
https://doi.org/10.1002/cpbi.3

Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids

Clotilde Théry, Sebastian Amigorena, Graça Raposo, Aled Clayton, 
https://doi.org/10.1002/0471143030.cb0322s30

ATAC‐seq: A Method for Assaying Chromatin Accessibility Genome‐Wide

Jason D. Buenrostro, Beijing Wu, Howard Y. Chang, William J. Greenleaf, 
https://doi.org/10.1002/0471142727.mb2129s109

Chlorophylls and Carotenoids: Measurement and Characterization by UV-VIS Spectroscopy

Hartmut K. Lichtenthaler, Claus Buschmann, 
https://doi.org/10.1002/0471142913.faf0403s01
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Using ggtree to Visualize Data on Tree‐Like Structures

Guangchuang Yu, 
https://doi.org/10.1002/cpbi.96

Using RepeatMasker to Identify Repetitive Elements in Genomic Sequences

Maja Tarailo-Graovac, Nansheng Chen, 
https://doi.org/10.1002/0471250953.bi0410s25

Best of Current Protocols

Using OrthoMCL to Assign Proteins to OrthoMCL‐DB Groups or to Cluster Proteomes Into New Ortholog Groups


Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids


Fluorescence Polarization (FP) Assays for Monitoring Peptide‐Protein or Nucleic Acid‐Protein Binding


Flow Cytometry Method Validation Protocols


Preparation, Culture, and Analysis of Amniotic Fluid Samples


Trypan Blue Exclusion Test of Cell Viability


ATAC‐seq: A Method for Assaying Chromatin Accessibility Genome‐Wide
Gram Staining


Cre‐loxP‐Mediated Recombination: General Principles and Experimental Considerations 


RNA‐Seq for Bacterial Gene Expression


Miniscope GRIN Lens System for Calcium Imaging of Neuronal Activity from Deep Brain Structures in Behaving Animals


Fixation and Laser Capture Microdissection of Plant Tissue for RNA Extraction and RNASeq Library Preparation


Streptozotocin‐Induced Diabetic Models in Mice and Rats


BioID: A Screen for Protein‐Protein Interactions


FACS‐Mediated Isolation of Neuronal Cell Populations From Virus‐Infected Human Embryonic Stem Cell-Derived Cerebral Organoid Cultures


Behavioral Phenotyping for Autism Spectrum Disorders in Mice


Zebrafish: Development of a Vertebrate Model Organism