25,000 step-by-step techniques, procedures, and practical overviews
Every Step Counts
The Current Protocols collection includes over 25,000 step-by-step techniques, procedures, and practical overviews that provide researchers with reliable, efficient methods to ensure reproducible results and pave the way for critical scientific discovery. With its emphasis on carefully curated, highly edited methods rich in detail, practical advice, and troubleshooting, Current Protocols enables researchers to advance their research with an efficiency of time and resources.
- Bioinformatics
- Cell Biology
- Chemical Biology
- Cytometry
- Essential Lab Techniques
- Food Analytical Chemistry
- Human Genetics
- Immunology
- Magnetic Resonance Imaging
- Microbiology
- Molecular Biology
- Mammalian Systems & Disease Models
- Neuroscience
- Nucleic Acid Chemistry
- Pharmacology
- Plant Biology
- Protein Science
- Stem Cell Biology
- Toxicology
Browse Articles
Visualization of the Cytoskeleton in Fixed Drosophila Embryos
- Current Protocols
-  15 May 2025
Navigating the Scholarly Literature: A Practical Guide to Searching Effectively (Without Too Much Stress)
- Current Protocols
-  9 May 2025
Highly Adaptable Analysis Tools for Mapping Spatial Features of Cellular Aggregates in Tissues
- Current Protocols
-  5 May 2025
Correction: Streaming Long‐Read Sequence Alignments for HLA Predictions Using HLAminer
- Current Protocols
-  5 May 2025
Purification of Low‐Complexity Domain Proteins FUS, EWSR1, and Their Fusions
- Current Protocols
-  26 April 2025
A Highly Sensitive Tissue‐specific qRT‐PCR‐based Assay for Detection of Melanoma Cells in Tumor‐Draining Lymph Nodes
- Current Protocols
-  26 April 2025
Enhancing Visual Discrimination Task: Optimized Mouse Motivation in the Touchscreen Paradigm
- Current Protocols
-  25 April 2025
Leveraging the MethMotif Toolkit to Characterize Context‐Specific Features and Roles of Methylation Sensitive Transcription Factors
- Current Protocols
-  25 April 2025
Protein Sequence Analysis Using the MPI Bioinformatics Toolkit
- Current Protocols in Bioinformatics
-  14 December 2020
Establishment of Humanized Mice from Peripheral Blood Mononuclear Cells or Cord Blood CD34+ Hematopoietic Stem Cells for Immune‐Oncology Studies Evaluating New Therapeutic Agents
- Current Protocols in Pharmacology
-  26 May 2020
An Optimized Method of Collecting Murine Peripheral Blood and Dilution Correction for Accurate Blood Cell Enumeration
- Current Protocols
-  26 May 2023
Flow Cytometry: An Overview
- Current Protocols in Immunology
-  5.1.1-5.1.11
-  21 February 2018
Measuring Mitochondrial Respiration in Previously Frozen Biological Samples
- Current Protocols in Cell Biology
-  15 December 2020
Perspectives on the Drosophila melanogaster Model for Advances in Toxicological Science
- Current Protocols
-  28 August 2023
Optimization of Large‐Scale Adeno‐Associated Virus (AAV) Production
- Current Protocols
-  11 May 2023
Sectioning and Counting of Motor Neurons in the L3 to L6 Region of the Adult Mouse Spinal Cord
- Current Protocols
-  26 May 2022
Design and Construction of a Designed Ankyrin Repeat Protein (DARPin) Display Library
- Current Protocols
-  11 January 2024
The GeneCards Suite: From Gene Data Mining to Disease Genome Sequence Analyses
- Current Protocols in Bioinformatics
-  1.30.1-1.30.33
-  20 June 2016
Gene Set Knowledge Discovery with Enrichr
- Current Protocols
-  29 March 2021
Using SPAdes De Novo Assembler
- Current Protocols in Bioinformatics
-  19 June 2020
From FastQ Data to High‐Confidence Variant Calls: The Genome Analysis Toolkit Best Practices Pipeline
- Current Protocols in Bioinformatics
-  11.10.1-11.10.33
-  15 March 2018
Comparative Protein Structure Modeling Using MODELLER
- Current Protocols in Bioinformatics
-  5.6.1-5.6.37
-  20 June 2016
Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
- Current Protocols in Cell Biology
-  3.22.1-3.22.29
-  1 April 2006
ATAC‐seq: A Method for Assaying Chromatin Accessibility Genome‐Wide
- Current Protocols in Molecular Biology
-  21.29.1-21.29.9
-  5 January 2015
Chlorophylls and Carotenoids: Measurement and Characterization by UV-VIS Spectroscopy
- Current Protocols in Food Analytical Chemistry
-  F4.3.1-F4.3.8
-  1 November 2001

Using RepeatMasker to Identify Repetitive Elements in Genomic Sequences
- Current Protocols in Bioinformatics
-  4.10.1-4.10.14
-  1 March 2009