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The Current Protocols collection includes over 25,000 step-by-step techniques, procedures, and practical overviews that provide researchers with reliable, efficient methods to ensure reproducible results and pave the way for critical scientific discovery. With its emphasis on carefully curated, highly edited methods rich in detail, practical advice, and troubleshooting, Current Protocols enables researchers to advance their research with an efficiency of time and resources.

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Scope Expansion: Call for Proposals Current Protocols in Mouse Biology

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PROTOCOL

Visualization of the Cytoskeleton in Fixed Drosophila Embryos

Junnan Fang, Jovan S. Brockett, Weiyi Tian, Yuqing Hua, Elizabeth R. Gavis, Dorothy A. Lerit, 
https://doi.org/10.1002/cpz1.70145
OVERVIEW
Open access

Navigating the Scholarly Literature: A Practical Guide to Searching Effectively (Without Too Much Stress)

Kathryn P. Kohn, 
https://doi.org/10.1002/cpz1.70138
PROTOCOL

Highly Effective Chemical Ligation of DNA and l-aTNA

Hikari Okita, Keiji Murayama, Hiroyuki Asanuma, 
https://doi.org/10.1002/cpz1.70140
PROTOCOL
Open access

Highly Adaptable Analysis Tools for Mapping Spatial Features of Cellular Aggregates in Tissues

Andrew Sawyer, Nick Weingaertner, Ellis Patrick, Carl G. Feng, 
https://doi.org/10.1002/cpz1.70135
CORRECTION
free access

Correction: Streaming Long‐Read Sequence Alignments for HLA Predictions Using HLAminer

René L. Warren, Inanc Birol, 
https://doi.org/10.1002/cpz1.70144
No abstract is available for this article.
PROTOCOL
Open access

Purification of Low‐Complexity Domain Proteins FUS, EWSR1, and Their Fusions

Jesse J. Altemus, Michelle A. Lay, Valery F. Thompson, Jacob C. Schwartz, 
https://doi.org/10.1002/cpz1.70136
PROTOCOL

A Highly Sensitive Tissue‐specific qRT‐PCR‐based Assay for Detection of Melanoma Cells in Tumor‐Draining Lymph Nodes

Kristian M. Hargadon, Travis B. Goodloe, 
https://doi.org/10.1002/cpz1.70139
PROTOCOL
Open access

Enhancing Visual Discrimination Task: Optimized Mouse Motivation in the Touchscreen Paradigm

Jeremy Jehl, Fabrice Riet, Aline Simonet, Yann Herault, 
https://doi.org/10.1002/cpz1.70126
PROTOCOL
Open access

Leveraging the MethMotif Toolkit to Characterize Context‐Specific Features and Roles of Methylation Sensitive Transcription Factors

Matthew Dyer, Gastongay Siu, Denis Thieffry, Touati Benoukraf, 
https://doi.org/10.1002/cpz1.70129
PROTOCOL
Open access

Cryptococcus neoformans Biofilm Formation and Quantification

Oscar Romero, Davier Gutierrez-Gongora, Jennifer Geddes-McAlister, 
https://doi.org/10.1002/cpz1.70133
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Open access

A Mouse Model of MC903‐Induced Atopic Dermatitis

Md Jahangir Alam, Liang Xie, Yu-Anne Yap, Remy Robert, 
https://doi.org/10.1002/cpz1.695
Open access

Protein Sequence Analysis Using the MPI Bioinformatics Toolkit

Felix Gabler, Seung-Zin Nam, Sebastian Till, Milot Mirdita, Martin Steinegger, Johannes Söding, Andrei N. Lupas, Vikram Alva, 
https://doi.org/10.1002/cpbi.108
Open access

Establishment of Humanized Mice from Peripheral Blood Mononuclear Cells or Cord Blood CD34+ Hematopoietic Stem Cells for Immune‐Oncology Studies Evaluating New Therapeutic Agents

Bhavna Verma, Amy Wesa, 
https://doi.org/10.1002/cpph.77
Open access

An Optimized Method of Collecting Murine Peripheral Blood and Dilution Correction for Accurate Blood Cell Enumeration

Vijay Bhoopalan, Elizabeth E. Gardiner, Amandeep Kaur, 
https://doi.org/10.1002/cpz1.765

Flow Cytometry: An Overview

Katherine M. McKinnon, 
https://doi.org/10.1002/cpim.40
Open access

Measuring Mitochondrial Respiration in Previously Frozen Biological Samples

Corey Osto, Ilan Y. Benador, Jennifer Ngo, Marc Liesa, Linsey Stiles, Rebeca Acin-Perez, Orian S. Shirihai, 
https://doi.org/10.1002/cpcb.116
Open access

Perspectives on the Drosophila melanogaster Model for Advances in Toxicological Science

Matthew D. Rand, Jason M. Tennessen, Trudy F. C. Mackay, Robert R. H. Anholt, 
https://doi.org/10.1002/cpz1.870
Open access

Optimization of Large‐Scale Adeno‐Associated Virus (AAV) Production

Alina S. Bilal, Sarah N. Parker, Victoria B. Murray, Lauren F. MacDonnell, Donna J. Thuerauf, Christopher C. Glembotski, Erik A. Blackwood, 
https://doi.org/10.1002/cpz1.757
Open access

Sectioning and Counting of Motor Neurons in the L3 to L6 Region of the Adult Mouse Spinal Cord

Adele Austin, Lynn Beresford, Georgia Price, Tom Cunningham, Bernadett Kalmar, Marianne Yon, 
https://doi.org/10.1002/cpz1.428
Open access

Design and Construction of a Designed Ankyrin Repeat Protein (DARPin) Display Library

Marco Morselli, Thomas R. Holton, Matteo Pellegrini, Todd O. Yeates, Mark A. Arbing, 
https://doi.org/10.1002/cpz1.960

The GeneCards Suite: From Gene Data Mining to Disease Genome Sequence Analyses

Gil Stelzer, Naomi Rosen, Inbar Plaschkes, Shahar Zimmerman, Michal Twik, Simon Fishilevich, Tsippi Iny Stein, Ron Nudel, Iris Lieder, Yaron Mazor, Sergey Kaplan, Dvir Dahary, David Warshawsky, Yaron Guan-Golan, Asher Kohn, Noa Rappaport, Marilyn Safran, Doron Lancet, 
https://doi.org/10.1002/cpbi.5

Gene Set Knowledge Discovery with Enrichr

Zhuorui Xie, Allison Bailey, Maxim V. Kuleshov, Daniel J. B. Clarke, John E. Evangelista, Sherry L. Jenkins, Alexander Lachmann, Megan L. Wojciechowicz, Eryk Kropiwnicki, Kathleen M. Jagodnik, Minji Jeon, Avi Ma'ayan, 
https://doi.org/10.1002/cpz1.90

Using SPAdes De Novo Assembler

Andrey Prjibelski, Dmitry Antipov, Dmitry Meleshko, Alla Lapidus, Anton Korobeynikov, 
https://doi.org/10.1002/cpbi.102

From FastQ Data to High‐Confidence Variant Calls: The Genome Analysis Toolkit Best Practices Pipeline

Geraldine A. Van der Auwera, Mauricio O. Carneiro, Christopher Hartl, Ryan Poplin, Guillermo del Angel, Ami Levy-Moonshine, Tadeusz Jordan, Khalid Shakir, David Roazen, Joel Thibault, Eric Banks, Kiran V. Garimella, David Altshuler, Stacey Gabriel, Mark A. DePristo, 
https://doi.org/10.1002/0471250953.bi1110s43

Comparative Protein Structure Modeling Using MODELLER

Benjamin Webb, Andrej Sali, 
https://doi.org/10.1002/cpbi.3

Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids

Clotilde Théry, Sebastian Amigorena, Graça Raposo, Aled Clayton, 
https://doi.org/10.1002/0471143030.cb0322s30

ATAC‐seq: A Method for Assaying Chromatin Accessibility Genome‐Wide

Jason D. Buenrostro, Beijing Wu, Howard Y. Chang, William J. Greenleaf, 
https://doi.org/10.1002/0471142727.mb2129s109

Chlorophylls and Carotenoids: Measurement and Characterization by UV-VIS Spectroscopy

Hartmut K. Lichtenthaler, Claus Buschmann, 
https://doi.org/10.1002/0471142913.faf0403s01
First page image

Using RepeatMasker to Identify Repetitive Elements in Genomic Sequences

Maja Tarailo-Graovac, Nansheng Chen, 
https://doi.org/10.1002/0471250953.bi0410s25

Dextran Sulfate Sodium (DSS)‐Induced Colitis in Mice

Benoit Chassaing, Jesse D. Aitken, Madhu Malleshappa, Matam Vijay-Kumar, 
https://doi.org/10.1002/0471142735.im1525s104

Best of Current Protocols

Using OrthoMCL to Assign Proteins to OrthoMCL‐DB Groups or to Cluster Proteomes Into New Ortholog Groups


Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids


Fluorescence Polarization (FP) Assays for Monitoring Peptide‐Protein or Nucleic Acid‐Protein Binding


Flow Cytometry Method Validation Protocols


Preparation, Culture, and Analysis of Amniotic Fluid Samples


Trypan Blue Exclusion Test of Cell Viability


ATAC‐seq: A Method for Assaying Chromatin Accessibility Genome‐Wide
Gram Staining


Cre‐loxP‐Mediated Recombination: General Principles and Experimental Considerations 


RNA‐Seq for Bacterial Gene Expression


Miniscope GRIN Lens System for Calcium Imaging of Neuronal Activity from Deep Brain Structures in Behaving Animals


Fixation and Laser Capture Microdissection of Plant Tissue for RNA Extraction and RNASeq Library Preparation


Streptozotocin‐Induced Diabetic Models in Mice and Rats


BioID: A Screen for Protein‐Protein Interactions


FACS‐Mediated Isolation of Neuronal Cell Populations From Virus‐Infected Human Embryonic Stem Cell-Derived Cerebral Organoid Cultures


Behavioral Phenotyping for Autism Spectrum Disorders in Mice


Zebrafish: Development of a Vertebrate Model Organism