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Adeno-Associated Viral Vectors for Anterograde Axonal Tracing with Fluorescent Proteins in Nontransgenic and Cre Driver Mice
Julie A. Harris,
Seung Wook Oh,
Hongkui Zeng,
Julie A. Harris
Allen Institute for Brain Science, Seattle, Washington
Search for more papers by this authorSeung Wook Oh
Allen Institute for Brain Science, Seattle, Washington
Search for more papers by this authorHongkui Zeng
Allen Institute for Brain Science, Seattle, Washington
Search for more papers by this authorJulie A. Harris,
Seung Wook Oh,
Hongkui Zeng,
Julie A. Harris
Allen Institute for Brain Science, Seattle, Washington
Search for more papers by this authorSeung Wook Oh
Allen Institute for Brain Science, Seattle, Washington
Search for more papers by this authorHongkui Zeng
Allen Institute for Brain Science, Seattle, Washington
Search for more papers by this authorAbstract
Harnessing the natural ability of viruses to infect post-mitotic cells such as neurons has provided an explosion of new methods to manipulate and reconstruct neural circuits in vivo. Here we describe the use of recombinant adeno-associated viral vectors (rAAV) for axonal tract tracing in nontransgenic and transgenic Cre driver mice. Two protocols are presented for stereotactic-guided placement of rAAV vectors into the live mouse brain using iontophoretic or nanoliter pressure injections. The methods discussed here will result in expression of fluorescent proteins in cell bodies, dendrites, and axons in targeted neurons, and can be easily adapted to address various experimental questions. Curr. Protoc. Neurosci. 59:1.20.1-1.20.18. © 2012 by John Wiley & Sons, Inc.
Literature Cited
-
Atasoy, D.,
Aponte, Y.,
Su, H.H., and
Sternson, S.M.
2008.
A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range circuit mapping.
J. Neurosci.
28: 7025-7030.
-
Bennett, J.,
Duan, D.,
Engelhardt, J.F., and
Maguire, A.M.
1997.
Real-time, noninvasive in vivo assessment of adeno-associated virus-mediated retinal transduction.
Invest. Ophthalmol. Vis. Sci.
38: 2857-2863.
-
Burger, C.,
Gorbatyuk, O.S.,
Velardo, M.J.,
Peden, C.S.,
Williams, P.,
Zolotukhin, S.,
Reier, P.J.,
Mandel, R.J., and
Muzyczka, N.
2004.
Recombinant AAV viral vectors pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism after delivery to different regions of the central nervous system.
Mol. Ther.
10: 302-317.
-
Cearley, C.N. and
Wolfe, J.H.
2007.
A single injection of an adeno-associated virus vector into nuclei with divergent connections results in widespread vector distribution in the brain and global correction of a neurogenetic disease.
J. Neurosci.
27: 9928-9940.
-
Cearley, C.N.,
Vandenberghe, L.H.,
Parente, M.K.,
Carnish, E.R.,
Wilson, J.M., and
Wolfe, J.H.
2008.
Expanded repertoire of AAV vector serotypes mediate unique patterns of transduction in mouse brain.
Mol. Ther.
16: 1710-1718.
-
Chamberlin, N.L.,
Du, B.,
de Lacalle, S., and
Saper, C.B.
1998.
Recombinant adeno-associated virus vector: Use for transgene expression and anterograde tract tracing in the CNS.
Brain Res.
793: 169-175.
-
Dhillon, H.,
Zigman, J.M.,
Ye, C.,
Lee, C.E.,
McGovern, R.A.,
Tang, V.,
Kenny, C.D.,
Christiansen, L.M.,
White, R.D.,
Edelstein, E.A.,
Coppari, R.,
Balthasar, N.,
Cowley, M.A.,
Chua, S. Jr.,
Elmquist, J.K., and
Lowell, B.B.
2006.
Leptin directly activates SF1 neurons in the VMH, and this action by leptin is required for normal body-weight homeostasis.
Neuron
49: 191-203.
-
Gautron, L.,
Lazarus, M.,
Scott, M.M.,
Saper, C.B., and
Elmquist, J.K.
2010.
Identifying the efferent projections of leptin-responsive neurons in the dorsomedial hypothalamus using a novel conditional tracing approach.
J. Comp. Neurol.
518: 2090-2108.
-
Hadaczek, P.,
Eberling, J.L.,
Pivirotto, P.,
Bringas, J.,
Forsayeth, J., and
Bankiewicz, K.S.
2010.
Eight years of clinical improvement in MPTP-lesioned primates after gene therapy with AAV2-hAADC.
Mol. Ther.
18: 1458-1461.
-
Kravitz, A.V.,
Freeze, B.S.,
Parker, P.R.,
Kay, K.,
Thwin, M.T.,
Deisseroth, K., and
Kreitzer, A.C.
2010.
Regulation of parkinsonian motor behaviours by optogenetic control of basal ganglia circuitry.
Nature
466: 622-626.
-
Kuhlman, S.J. and
Huang, Z.J.
2008.
High-resolution labeling and functional manipulation of specific neuron types in mouse brain by Cre-activated viral gene expression.
PLoS One
3: e2005.
-
Madisen, L.,
Zwingman, T.A.,
Sunkin, S.M.,
Oh, S.W.,
Zariwala, H.A.,
Gu, H.,
Ng, L.L.,
Palmiter, R.D.,
Hawrylycz, M.J.,
Jones, A.R.,
Lein, E.S., and
Zeng, H.
2010.
A robust and high-throughput Cre reporting and characterization system for the whole mouse brain.
Nat. Neurosci.
13: 133-140.
-
Marik, S.A.,
Yamahachi, H.,
McManus, J.N.,
Szabo, G., and
Gilbert, C.D.
2010.
Axonal dynamics of excitatory and inhibitory neurons in somatosensory cortex.PLoS Biol.
8: e100395.
-
Mittmann, W.,
Wallace, D.J.,
Czubayko, U.,
Herb, J.T.,
Schaefer, A.T.,
Looger, L.L.,
Denk, W., and
Kerr, J.N.
2011.
Two-photon calcium imaging of evoked activity from L5 somatosensory neurons in vivo.
Nat. Neurosci.
14: 1089-1093.
-
Passini, M.A.,
Watson, D.J.,
Vite, C.H.,
Landsburg, D.J.,
Feigenbaum, A.L., and
Wolfe, J.H.
2003.
Intraventricular brain injection of adeno-associated virus type 1 (AAV1) in neonatal mice results in complementary patterns of neuronal transduction to AAV2 and total long-term correction of storage lesions in the brains of beta-glucuronidase-deficient mice.
J. Virol.
77: 7034-7040.
-
Paxinos, G. and
Franklin, K.B.J.
2001.
The mouse brain in stereotaxic coordinates
( 2nd edition).
Academic Press,
New York.
-
Ragan, T.,
Kadiri, L.R.,
Venkataraju, K.U.,
Bahlmann, K.,
Sutin, J.,
Taranda, J.,
Arganda-Carreras, I.,
Kim, Y.,
Seung, H.S., and
Osten, P.
2012.
Serial two-photon tomography for automated ex vivo mouse brain imaging.
Nat. Methods
9: 255-258.
-
Spires, T.L.,
Meyer-Luehmann, M.,
Stern, E.A.,
McLean, P.J.,
Skoch, J.,
Nguyen, P.T.,
Bacskai, B.J., and
Hyman, B.T.
2005.
Dendritic spine abnormalities in amyloid precursor protein transgenic mice demonstrated by gene transfer and intravital multiphoton microscopy.
J. Neurosci.
25: 7278-7287.
-
Sternson, S. and
Betley, J.N.
2011.
Adeno-associated viral vectors for mapping, monitoring, and manipulating neural circuits.
Hum. Gene Ther.
-
Stettler, D.D.,
Yamahachi, H.,
Li, W.,
Denk, W., and
Gilbert, C.D.
2006.
Axons and synaptic boutons are highly dynamic in adult visual cortex.
Neuron
49: 877-887.
-
Taymans, J.M.,
Vandenberghe, L.H.,
Haute, C.V.,
Thiry, I.,
Deroose, C.M.,
Mortelmans, L.,
Wilson, J.M.,
Debyser, Z., and
Baekelandt, V.
2007.
Comparative analysis of adeno-associated viral vector serotypes 1, 2, 5, 7, and 8 in mouse brain.
Hum. Gene Ther.
18: 195-206.
-
Van Vliet, K.M.,
Blouin, V.,
Brument, N.,
Agbandje-McKenna, M., and
Snyder, R.O.
2008.
The role of the adeno-associated virus capsid in gene transfer.
Methods Mol. Biol.
437: 51-91.
-
Vandenberghe, L.H.,
Breous, E.,
Nam, H.J.,
Gao, G.,
Xiao, R.,
Sandhu, A.,
Johnston, J.,
Debyser, Z.,
Agbandje-McKenna, M., and
Wilson, J.M.
2009.
Naturally occurring singleton residues in AAV capsid impact vector performance and illustrate structural constraints.
Gene Ther.
16: 1416-1428.
-
Wu, Z.,
Asokan, A., and
Samulski, R.J.
2006.
Adeno-associated virus serotypes: Vector toolkit for human gene therapy.
Mol. Ther.
14: 316-327.
-
Xiao, X.,
Li, J.,
McCown, T.J., and
Samulski, R.J.
1997.
Gene transfer by adeno-associated virus vectors into the central nervous system.
Exp. Neurol.
144: 113-124.
