Volume 20, Issue 1 p. 9A.2.1-9A.2.17
UNIT

Genetic Manipulation of Clostridium difficile

Firmicutes (Low G+C Gram Positive)

Laurent Bouillaut

Laurent Bouillaut

Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts

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Shonna M. McBride

Shonna M. McBride

Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts

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Joseph A. Sorg

Joseph A. Sorg

Department of Biology, Texas A & M University, College Station, Texas

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First published: 15 February 2011
Citations: 48

ABSTRACT

Clostridium difficile is a Gram-positive, spore forming, anaerobic, intestinal bacterium and is the most common cause of antibiotic-associated colitis. For many years this organism was considered genetically intractable, but in the past 10 years, multiple methods have been developed or adapted for genetic manipulation of C. difficile. This unit describes the molecular techniques used for genetic modification of this organism, including methods for gene disruption, complementation, plasmid introduction and integration, and cross-species conjugations.