Volume 71, Issue 1 p. 10.9.1-10.9.34
UNIT

Preparation of Extracellular Matrices Produced by Cultured and Primary Fibroblasts

Janusz Franco-Barraza

Janusz Franco-Barraza

Fox Chase Cancer Center, Philadelphia, Pennsylvania

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Dorothy A. Beacham

Dorothy A. Beacham

Fox Chase Cancer Center, Philadelphia, Pennsylvania

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Michael D. Amatangelo

Michael D. Amatangelo

Fox Chase Cancer Center, Philadelphia, Pennsylvania

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Edna Cukierman

Edna Cukierman

Fox Chase Cancer Center, Philadelphia, Pennsylvania

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First published: 01 June 2016
Citations: 94

Abstract

Fibroblasts secrete and organize extracellular matrix (ECM), which provides structural support for their adhesion, migration, and tissue organization, besides regulating cellular functions such as growth and survival. Cell-to-matrix interactions are vital for vertebrate development. Disorders in these processes have been associated with fibrosis, developmental malformations, cancer, and other diseases. This unit describes a method for preparing a three-dimensional matrix derived from fibroblastic cells; the matrix is three-dimensional, cell and debris free, and attached to a two-dimensional culture surface. Cell adhesion and spreading are normal on these matrices. This matrix can also be compressed into a two-dimensional matrix and solubilized to study the matrix biochemically. © 2016 by John Wiley & Sons, Inc.