Volume 44, Issue 1 p. 1F.21.1-1F.21.6
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Direct Conversion of Human Pluripotent Stem Cells to Osteoblasts With a Small Molecule

Heemin Kang

Heemin Kang

Department of Materials Science and Engineering, University of California, San Diego, La Jolla, California

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Yu-Ru V. Shih

Yu-Ru V. Shih

Department of Bioengineering, University of California, San Diego, La Jolla, California

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Shyni Varghese

Shyni Varghese

Department of Materials Science and Engineering, University of California, San Diego, La Jolla, California

Department of Bioengineering, University of California, San Diego, La Jolla, California

Department of Nanoengineering, University of California, San Diego, La Jolla, California

To whom correspondence should be addressed. E-mail: [email protected]

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First published: 28 February 2018
Citations: 9

Abstract

Human pluripotent stem cells (hPSCs), which exhibit unlimited self-renewal ability and can differentiate into all cell types in the human body, are a promising cell source for cell-based therapies and regenerative medicine. Small molecules hold great potential in the derivation of tissue-specific cells from hPSCs owing to their cost-effectiveness and scalability. Here, we describe a protocol for deriving osteoblasts from hPSCs by using a single, natural small molecule: adenosine. This simple and effective experimental protocol allows one to obtain large numbers of osteoblasts or osteoprogenitor cells, with the ability to form functional bone tissues, from hPSCs, including human embryonic stem cells and induced pluripotent stem cells. This protocol could potentially enable studies of tissue regeneration and skeletal diseases. © 2018 by John Wiley & Sons, Inc.