UNIT
Generation of Transgenic Mice
Abstract
This unit describes detailed step‐by‐step protocols, reagents, and equipment required for successful generation of transgenic mice using pronuclear injection. The experimental methods and practical tips given here will help guide beginners in understanding what is required and what to avoid in these standard protocols for efficiently generating transgenic mice. Curr. Protoc. Cell Biol. 42:19.11.1‐19.11.22. © 2009 by John Wiley & Sons, Inc.
Citing Literature
Number of times cited: 12
- Shang Wang and Irina V. Larina, In Vivo Imaging of the Mouse Reproductive Organs, Embryo Transfer, and Oviduct Cilia Dynamics Using Optical Coherence Tomography, Mouse Embryogenesis, 10.1007/978-1-4939-7714-7_5, (53-62), (2018).
- Marietta Landgraf, Jacqui A. McGovern, Peter Friedl and Dietmar W. Hutmacher, Rational Design of Mouse Models for Cancer Research, Trends in Biotechnology, 10.1016/j.tibtech.2017.12.001, 36, 3, (242-251), (2018).
- Sarah A. Stanley, Genetically Encoded Nanoparticles for Neural Modulation, Use of Nanoparticles in Neuroscience, 10.1007/978-1-4939-7584-6_5, (53-67), (2018).
- Colin E. J. Pritchard, Lona J. Kroese and Ivo J. Huijbers, Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes, Site-Specific Recombinases, 10.1007/978-1-4939-7169-5_2, (21-35), (2017).
- Wei-Hua Chen, Guanting Lu, Xiao Chen, Xing-Ming Zhao and Peer Bork, OGEE v2: an update of the online gene essentiality database with special focus on differentially essential genes in human cancer cell lines, Nucleic Acids Research, 10.1093/nar/gkw1013, 45, D1, (D940-D944), (2016).
- Lisette Johana Latorre Rey and Ute Modlich, Strategies for the Gene Modification of Megakaryopoiesis and Platelets, Molecular and Cellular Biology of Platelet Formation, 10.1007/978-3-319-39562-3_20, (421-460), (2017).
- Florian Douam, Jenna M. Gaska, Benjamin Y. Winer, Qiang Ding, Markus von Schaewen and Alexander Ploss, Genetic Dissection of the Host Tropism of Human-Tropic Pathogens, Annual Review of Genetics, 10.1146/annurev-genet-112414-054823, 49, 1, (21-45), (2015).
- Hitomi Tsuiji and Koji Yamanaka, Animal Models for Neurodegenerative Disorders, Animal Biotechnology, 10.1016/B978-0-12-416002-6.00003-1, (39-56), (2014).
- Mitchell E. Menezes, Swadesh K. Das, Luni Emdad, Jolene J. Windle, Xiang-Yang Wang, Devanand Sarkar and Paul B. Fisher, Genetically Engineered Mice as Experimental Tools to Dissect the Critical Events in Breast Cancer, , 10.1016/B978-0-12-800249-0.00008-1, (331-382), (2014).
- Thomas L. Platt, Valerie L. Reeves and M. Paul Murphy, Transgenic models of Alzheimer's disease: Better utilization of existing models through viral transgenesis, Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, 10.1016/j.bbadis.2013.04.017, 1832, 9, (1437-1448), (2013).
- Shin Watanabe, Takahiro Horie, Kazuya Nagao, Yasuhide Kuwabara, Osamu Baba, Hitoo Nishi, Naoya Sowa, Michiko Narazaki, Tetsuya Matsuda, Genzou Takemura, Hiromichi Wada, Koji Hasegawa, Takeshi Kimura, Koh Ono and Vincenzo Lionetti, Cardiac-Specific Inhibition of Kinase Activity in Calcium/Calmodulin-Dependent Protein Kinase Kinase-β Leads to Accelerated Left Ventricular Remodeling and Heart Failure after Transverse Aortic Constriction in Mice, PLoS ONE, 10.1371/journal.pone.0108201, 9, 9, (e108201), (2014).
- Andrew Cho, Naoto Haruyama, Bradford Hall, Mary Jo S. Danton, Lu Zhang, Praveen Arany, David J. Mooney, Yassine Harichane, Michel Goldberg, Carolyn W. Gibson, Ashok B. Kulkarni and Songtao Shi, TGF-ß Regulates Enamel Mineralization and Maturation through KLK4 Expression, PLoS ONE, 10.1371/journal.pone.0082267, 8, 11, (e82267), (2013).
