Modern biology is on the verge of officially ushering in a new era in science with the completion of the sequencing of the human genome in April 2003. While often erroneously called the “post‐genome era”, this will actually truly mark the beginning of the “genome era,” a time in which the availability of sequence data for many genomes will have a significant effect on how science is performed in the 21st century. This unit offers an overview of many of the gene prediction methods that are currently available and offers a general assessment of how well the methods work for various problems.