Human Papillomavirus Collection

9 April 2021
19 April 2022

The Editors of the Microbiology section of Current Protocols are pleased to bring you this special protocol-based virtual issue. This collection brings together protocols published since 2007 that are useful to researchers studying papillomaviruses.

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Table of Contents

Development of Keratinocyte Cell Lines Containing Extrachromosomal Human Papillomavirus Genomes

Tami L. Coursey,  Alison A. McBride, 

High-Throughput NanoString Analysis of Oncogenic Human Papillomavirus and Tumor Microenvironment Transcription in Head and Neck Squamous Cell Carcinoma

Wesley H. Stepp, 

3D Oral and Cervical Tissue Models for Studying Papillomavirus Host-Pathogen Interactions

Robert Jackson,  Jason D. Maarsingh,  Melissa M. Herbst-Kralovetz,  Koenraad Van Doorslaer, 

An Exonuclease V–qPCR Assay to Analyze the State of the Human Papillomavirus 16 Genome in Cell Lines and Tissues

Julia E. Myers,  Katarzyna Zwolinska,  Martin J. Sapp,  Rona S. Scott, 

Human Papillomavirus Quasivirus Production and Infection of Primary Human Keratinocytes

Samuel S. Porter,  Alison A. McBride, 

Building (Viral) Phylogenetic Trees Using a Maximum Likelihood Approach

Kelly M. King,  Koenraad Van Doorslaer, 

Human Papillomavirus Integration: Analysis by Molecular Combing and Fiber-FISH

Catherine J. Redmond,  Haiqing Fu,  Mirit I. Aladjem,  Alison A. McBride, 

Analysis of Human Papillomavirus Genome Replication Using Two- and Three-Dimensional Agarose Gel Electrophoresis

Liisi Henno,  Eva-Maria Tombak,  Jelizaveta Geimanen,  Marit Orav,  Ene Ustav,  Mart Ustav, 

Detection and Genotyping of Human Papillomaviruses from Archival Formalin-Fixed Tissue Samples

Koenraad Van Doorslaer,  Zigui Chen,  Alison A. McBride, 

Detection of Papillomavirus Gene Expression Patterns in Tissue Sections

Heather Griffin,  John Doorbar, 

Using Organotypic Epithelial Tissue Culture to Study the Human Papillomavirus Life Cycle

Denis Lee,  Kathryn Norby,  Mitchell Hayes,  Ya-Fang Chiu,  Bill Sugden,  Paul F. Lambert, 

Production of Furin-Cleaved Papillomavirus Pseudovirions and Their Use for In Vitro Neutralization Assays of L1- or L2-Specific Antibodies

Joshua W. Wang,  Ken Matsui,  Yuanji Pan,  Kihyuck Kwak,  Shiwen Peng,  Troy Kemp,  Ligia Pinto,  Richard B.S. Roden, 

Cell Culture Assay for Transient Replication of Human and Animal Papillomaviruses

Van G. Wilson, 

Fluorescently Labeled Human Papillomavirus Pseudovirions for Use in Virus Entry Experiments

Pilar Samperio Ventayol,  Mario Schelhaas, 

Construction of a Transcription Map for Papillomaviruses using RACE, RNase Protection, and Primer Extension Assays

Xiaohong Wang,  Zhi-Ming Zheng, 

Cell Culture Assay for Transient Replication of Human and Animal Papillomaviruses

Van G. Wilson, 

Using Organotypic (Raft) Epithelial Tissue Cultures for the Biosynthesis and Isolation of Infectious Human Papillomaviruses

Michelle A. Ozbun,  Nicole A. Patterson, 

Analyzing the Human Papillomavirus (HPV) Life Cycle in Primary Keratinocytes with a Quantitative Colony-Forming Assay

Michael J. Lace,  Lubomir P. Turek,  James R. Anson,  Thomas H. Haugen,