The Molecular Biology section of Current Protocols is a comprehensive source for step-by-step protocols and overviews covering essential and advanced experimental design, methods, and analyses in all areas of molecular biology, including the preparation and analysis of DNA, RNA and proteins, sequencing, genome editing, gene regulation and expression, chromatin assembly, and more.

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CRISPR/Cas9 Ribonucleoprotein Nucleofection for Genome Editing in Primary Human Keratinocytes: Knockouts, Deletions, and Homology‐Directed Repair Mutagenesis

Martina Bamundo, Sara Palumbo, Ludovica D'Auria, Caterina Missero, Daniela Di Girolamo, 

DMS‐MapSeq Analysis of Antisense Oligonucleotide Binding to lncRNA PANDA

Gabriel A. Romero Agosto, Ethan Cox, Silvi Rouskin, 
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Injection of Ortho-Functionalized Tetrafluorinated Azobenzene-Containing siRNAs into Japanese Medaka Embryos for Photocontrolled Gene Silencing

Makenzie Mateus, Matthew L. Hammill, Denina B. D. Simmons, Jean-Paul Desaulniers, 

Efficient CRISPR/Cas9 Knock‐in Approaches for Manipulation of Endogenous Genes in Human B Lymphoma Cells

Laura A. Murray-Nerger, Benjamin E. Gewurz, 

Genetic Modification of Mice Using Prime Editing

Amr R. Salem, Xiaoling Xie, Susan H. Griffin, Lin Gan, Joseph M Miano, 
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ATAC‐seq: A Method for Assaying Chromatin Accessibility Genome‐Wide

Jason D. Buenrostro, Beijing Wu, Howard Y. Chang, William J. Greenleaf, 
  •  21.29.1-21.29.9
  •  5 January 2015

Overview of Next‐Generation Sequencing Technologies

Barton E. Slatko, Andrew F. Gardner, Frederick M. Ausubel, 
  •  16 April 2018

Metabolomics by Gas Chromatography–Mass Spectrometry: Combined Targeted and Untargeted Profiling

Oliver Fiehn, 
  •  30.4.1-30.4.32
  •  1 April 2016
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