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    Current Protocols: Volume 4, Issue 11

    November 2024
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ISSUE INFORMATION

Free Access

Issue Information

  • First Published: 27 November 2024

FEATURED COVER

Free Access

Cover Image, Volume 4, Issue 11

  • First Published: 27 November 2024
Cover Image, Volume 4, Issue 11 Volume 4 Issue 11, 2024

The cover image is based on the Article PhyKIT: A Multitool for Phylogenomics by Jacob L. Steenwyk et al., https://doi.org/10.1002/cpz1.70016.

PROTOCOL

Open Access

Analysis of Tandem Repeats in Short-Read Sequencing Data: From Genotyping Known Pathogenic Repeats to Discovering Novel Expansions

Andreas Halman,  Andrew Lonsdale,  Alicia Oshlack, 
  • First Published: 05 November 2024
Open Access

PhyKIT: A Multitool for Phylogenomics

Jacob L. Steenwyk,  Gemma I. Martínez-Redondo,  Thomas J. Buida III,  Emile Gluck-Thaler,  Xing-Xing Shen,  Toni Gabaldón,  Antonis Rokas,  Rosa Fernández, 
  • First Published: 30 October 2024
Open Access

Isolation of Small Extracellular Vesicles (sEVs) from the Apoplastic Wash Fluid of Nicotiana benthamiana Leaves

Mahmoud K. Eldahshoury,  Konstantina Katsarou,  Joshua T. Farley,  Kriton Kalantidis,  Carine de Marcos Lousa, 
  • First Published: 05 November 2024
Open Access

Transmission Electron Microscopy of Coral Tissue

Erin Papke,  Grace E. Kennedy,  Elizabeth Elliott,  Alison Taylor,  Bradley B. Tolar,  Blake Ushijima, 
  • First Published: 30 October 2024

DMS-MapSeq Analysis of Antisense Oligonucleotide Binding to lncRNA PANDA

Gabriel A. Romero Agosto,  Ethan Cox,  Silvi Rouskin, 
  • First Published: 26 November 2024

Optimizing CAR-NK Cell Transduction and Expansion: Leveraging Cytokine Modulation for Enhanced Performance

Tiziano Ingegnere,  Benjamin Segain,  Adeline Cozzani,  Mattias Carlsten,  Suman Mitra,  Silvia Gaggero, 
  • First Published: 30 October 2024

Efficient CRISPR/Cas9 Knock-in Approaches for Manipulation of Endogenous Genes in Human B Lymphoma Cells

Laura A. Murray-Nerger,  Benjamin E. Gewurz, 
  • First Published: 13 November 2024

Automatic Experimental Numerosity Generation and Numerical Training for Rodents

Tuo Liang,  Kang-Lin Rong,  Jing-Da Qiao,  Ya Ke,  Wing-Ho Yung, 
  • First Published: 12 November 2024

Human Pancreas-Derived Organoids with Controlled Polarity: Detailed Protocols and Experimental Timeline

Aletta Kiss,  Attila Farkas,  Ferhan Ayaydin,  György Lázár,  Árpád Varga,  József Maléth, 
  • First Published: 26 November 2024
Open Access

Analyzing Extracellular Vesicle-associated DNA Using Transmission Electron Microscopy at the Single EV-level

Thupten Tsering,  Amélie Nadeau,  Janusz Rak,  Julia V. Burnier, 
  • First Published: 08 November 2024

Quantification of Sarcoplasmic Reticulum Ca2+ Release in Primary Ventricular Cardiomyocytes

Md Nure Alam Afsar,  Mahmuda Akter,  Christopher Y. Ko,  Vasco Sequeira,  Yusuf Olgar,  Christopher N. Johnson, 
  • First Published: 30 October 2024

Generating iAstrocytes From Human Induced Pluripotent Stem Cells by Combining Low-Density Passaging of Neural Progenitor Cells and Transcription Factor NFIA Transdifferentiation

Patrick Bosco,  Ugur Akcan,  Damian Williams,  Heather M. Buchanan,  Dritan Agalliu,  Andrew A. Sproul, 
  • First Published: 15 November 2024
Open Access

Injection of Ortho-Functionalized Tetrafluorinated Azobenzene-Containing siRNAs into Japanese Medaka Embryos for Photocontrolled Gene Silencing

Makenzie Mateus,  Matthew L. Hammill,  Denina B. D. Simmons,  Jean-Paul Desaulniers, 
  • First Published: 15 November 2024

Analysis of Free Oligosaccharides in Urine by High-Performance Liquid Chromatography–Tandem Mass Spectrometry

J. Daniel Sharer,  Rongrong Huang,  Timothy Wood,  Paula Huffman,  Yang Yan,  Chelsea Zimmerman,  Laura Pollard, 
  • First Published: 22 November 2024
Open Access

CRISPR/Cas9 Ribonucleoprotein Nucleofection for Genome Editing in Primary Human Keratinocytes: Knockouts, Deletions, and Homology-Directed Repair Mutagenesis

Martina Bamundo,  Sara Palumbo,  Ludovica D'Auria,  Caterina Missero,  Daniela Di Girolamo, 
  • First Published: 27 November 2024

Engineering and Evaluating Vascularized Organotypic Spheroids On-Chip

James J. Tronolone,  Nadin Mohamed,  Christopher P. Chaftari,  Yuxiang Sun,  Tanmay Mathur,  Abhishek Jain, 
  • First Published: 21 November 2024
Open Access

Workflow to Select Functional Promoter DNA Baits and Screen Arrayed Gene Libraries in Yeast

Iris Fañanás-Pueyo,  Ana-Mariya Anhel,  Ángel Goñi-Moreno,  Luis Oñate-Sánchez,  Gerardo Carrera-Castaño, 
  • First Published: 21 November 2024
Open Access

Vesicular Stomatitis Virus as a Platform for Protease Activity Measurements

Stefanie Rauch,  Francesco Costacurta,  Dorothee von Laer,  Emmanuel Heilmann, 
  • First Published: 21 November 2024
Open Access

Multi-site Ultrasound-guided Fine Needle Aspiration to Study Cells and Soluble Factors From Human Lymph Nodes

Adam Al-Diwani,  Deepsha Agrawal,  Fintan Sheerin,  Callum Board,  Sarosh R. Irani,  Katrina M. Pollock,  Nicholas M. Provine, 
  • First Published: 23 November 2024

PROTOCOL COMMUNICATION

NeuroClick: Advanced Software for Designing Blood−Brain Barrier-Permeable Drugs Using Click Reaction Simulations

Anastasiia M. Isakova,  Ekaterina V. Skorb,  Sergey Shityakov, 
  • First Published: 08 November 2024

Synthesis and Application of a Caged Bioluminescent Probe for the Immunoproteasome

Cody A. Loy,  Darci J. Trader, 
  • First Published: 22 November 2024

UPDATED PROTOCOL

Using the RNAstructure Software Package to Predict Conserved RNA Structures

Abhinav Mittal,  Sara E. Ali,  David H. Mathews, 
  • First Published: 14 November 2024

CORRECTION

Free Access

Correction: Recombineering: Genetic Engineering in Escherichia coli Using Homologous Recombination

Lynn C. Thomason,  Nina Costantino,  Xintian Li,  Donald L. Court, 
  • First Published: 14 November 2024